Where is protein sequence in UniProt?

Where is protein sequence in UniProt?

Retrieving sequences from the website

  1. Perform your favorite query and view the resulting list of entries (e.g. this query retrieves all UniProtKB entries that are part of the human proteome: proteome:UP000005640)
  2. Click the Download button in the query result page.

Is UniProt a protein database?

UniProt is a freely accessible database of protein sequence and functional information, many entries being derived from genome sequencing projects. It contains a large amount of information about the biological function of proteins derived from the research literature.

How many protein sequences are in UniProt?

UniProtKB/Swiss-Prot has curated over 81 000 variants in 13,000 human protein sequence records from peer-reviewed literature, annotated with information on the functional impact and clinical significance, when known.

Where can I find protein sequence?

The protein sequence can also be found by clicking on the protein accession number in the Nucleotide record or in the RefSeq section of the Gene record.

What is the difference between Swiss-Prot and UniProt?

UniProt provides a comprehensive, high-quality and freely accessible resource of protein sequence and functional information. UniProtKB/TrEMBL is a computer-annotated (unreviewed) supplement to Swiss-Prot, which strives to gather all protein sequences that are not yet represented in Swiss-Prot.

Why do we need UniProt?

UniProt helps with this in the following ways: It provides an up-to-date, comprehensive body of protein information at a single site. It aids scientific discovery by collecting, interpreting and organising this information so that it is easy to access and use. It provides tools to help with protein sequence analysis.

Why is UniProt used?

The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data. EMBL-EBI and SIB together used to produce Swiss-Prot and TrEMBL, while PIR produced the Protein Sequence Database (PIR-PSD). …

Where is protein sequence in Uniprot?

Retrieving sequences from the website

  1. Perform your favorite query and view the resulting list of entries (e.g. this query retrieves all UniProtKB entries that are part of the human proteome: proteome:UP000005640)
  2. Click the Download button in the query result page.

What is the function of SIRT1?

SIRT1 protein protects the functions of adipose tissue and liver in several aspects (29, 30) such as glucose homeostasis and fat metabolism against severe obesity (31, 32). It is also involved in energy balance and stress.

How can I increase my SIRT1?

Levels of Sirt6 are increased upon nutrient deprivation in cultured cells. Sirt6, like Sirt1 levels, are also increased in the brain, kidney and heart of mice after a 24hr fasting period, and in white adipose tissue (WAT), heart and brain of rats fed a calorie-restricted diet for a minimum of one year (Kanfi, et al.

What is sirtuin activity?

Sirtuins’ activity is linked to metabolic control, apoptosis, cell survival, development, inflammation, and healthy aging. They act as sensors of energy and redox status in cells; they modulate the activity of key metabolic enzymes as well as regulate transcription of metabolic genes.

Where is the amino acid sequence of a protein found?

The sequence of a protein is usually notated as a string of letters, according to the order of the amino acids from the amino-terminal to the carboxyl-terminal of the protein. Either a single or three-letter code may be used to represent each amino acid in the sequence.

How do you find the similarity of proteins?

Select the Blast tab of the toolbar to run a sequence similarity search with the BLAST (Basic Local Alignment Search Tool) program: Enter either a protein or nucleotide sequence (raw sequence or fasta format) or a UniProt identifier into the form field. Click the Blast button.

What does SIRT1 stand for?

SIRT1 stands for sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), referring to the fact that its sirtuin homolog (biological equivalent across species) in yeast (Saccharomyces cerevisiae) is Sir2.

How is SIRT1 activated?

Oxidative stress could activate SIRT1 signaling pathways by regulating AMPK and/or directly via SIRT1. Indeed, while some authors demonstrate that oxidative stress decreases SIRT1 activity [154,155], other authors suggest that oxidative stress induces an increase in the expression of SIRT1 [156,157].

Does fasting increase sirtuins?

Sirtuins, particularly SIRT1 and SIRT3, can be activated by fasting and further exhibit their effects in insulin response, antioxidant defense, and glycolysis. Therefore, sirtuins may have anticancer effects by shifting metabolism to a less proliferative cell phenotype as well as less prone to oxidative stress attack.

How does deacetylase in sirtuin affect gene expression?

Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively (PubMed: 15152190, PubMed: 14980222, PubMed: 14976264 ).

How does the UniProt basket work in grxc3?

When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later. More… The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome.

How does deacetylase SREBF1 affect lipogenic gene expression?

Proposed to deacetylate IRS2 thereby facilitating its insulin-induced tyrosine phosphorylation. Deacetylates SREBF1 isoform SREBP-1C thereby decreasing its stability and transactivation in lipogenic gene expression (PubMed: 17290224, PubMed: 20817729 ).