What does THP-1 stand for?
Rating. THP1. Tamm-Horsfall Protein 1.
What are THP-1 macrophages?
THP-1 cells can be differentiated into macrophage-like cells that resemble properties of mature macrophages by activation of protein kinase C (PKC) with phorbol-12-myristate-13-acetate (PMA), ultimately resulting in cells with increased adherence and loss of proliferative activity (Schwende et al., 1996).
Are THP-1 cells adherent or suspension?
Normally THP-1 cells grow in suspension, but they can be induced to differentiate into adherent macrophage-like cells, which express IL-8 and other markers, and can thus serve as a model system for differentiation.
How do you thaw THP-1 cells?
To Successfully Freeze THP-1 Cells, Follow These Tips: Thaw frozen vials in a 37°C water bath, but DO NOT LET THE WATER COME IN CONTACT WITH THE LID. Thaw the contents until there’s a small lump of ice in the bottom—trust me, it may seem counter-intuitive, but it works.
What type of cells are THP-1?
THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.
Where did THP-1 cells come from?
In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglobulins.
How do you freeze THP-1 cells?
To Successfully Freeze THP-1 Cells, Follow These Tips:
- Thaw frozen vials in a 37°C water bath, but DO NOT LET THE WATER COME IN CONTACT WITH THE LID.
- Transfer the cells to a flask containing warmed media, then transfer some of this back into the vial to get as many cells as you can.
How do you freeze down a THP-1 cell?
How is THP-1 sensitive to culture conditions?
Understanding their biology becomes imperative when trying to elucidate immune cell interactions with their environment, and in vitro cell lines allow researchers to manipulate these interactions. A common cell line used is THP-1, a promyeloid cell line suggestive to outside factors, and therefore sensitive to culture conditions.
How are THP-1 cells differentiated in vitro?
In vitro models of differing macrophage functions are useful since human monocyte–derived macrophages are short–lived, finite and vary from donor to donor. Published protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS.
How does culture condition affect THP-1 response to PMA?
Results: Culture conditions of THP-1 affect their response to PMA. Highly confluent THP-1 cells differentiate into a heterogeneous population responsive to PMA as seen by an increase in CD14 expression. However, these cells, cultured in low confluence, remain as a homogenous population and do not gain CD14.
Which is the best treatment for THP-1 cells?
Treatment of THP-1 cells with PMA as a priming step, followed by rest and treatment with either proinflammatory or regulatory cytokines results in macrophage-like cells that express some of the marker genes typical of human monocyte-derived macrophages (hMDMs) ( Daigneault et al., 2010; Kohro et al., 2004; Shiratori et al., 2017 ).