What is oligonucleotide microarray?
Oligo DNA microarrays. (3D-Gene®） An oligo DNA microarray is a DNA microarray whose probe is the chemically synthesized oligo DNA after genes corresponding to exons in the region of 20-100 nts are selected.
How does oligonucleotide microarray work?
Oligonucleotide arrays are produced by printing short oligonucleotide sequences designed to represent a single gene or family of gene splice-variants by synthesizing this sequence directly onto the array surface instead of depositing intact sequences.
What are expression arrays?
A gene expression array (or DNA microarray) is a miniature, massively parallel, and semi-automated process that measures levels of gene products (or mRNA or transcripts). A genetic message is transcribed from DNA into mRNA (messenger RNA), then typically translated into protein.
What advantage do oligonucleotide arrays have over cDNA arrays?
Oligonucleotide-based microarrays offer a number of advantages over cDNA microarrays, including (i) more controlled specificity of hybridization, which makes them particularly useful for the analysis of single nucleotide polymorphisms (17) or mutational analysis (18,19); (ii) versatility to address subtle questions …
What is microarray used for?
A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. DNA microarrays are microscope slides that are printed with thousands of tiny spots in defined positions, with each spot containing a known DNA sequence or gene.
What microarray means?
Listen to pronunciation. (MY-kroh-uh-RAY) A laboratory tool used to analyze large numbers of genes or proteins at one time. In a microarray, biologic molecules such as DNA, RNA, or protein are placed in a pattern onto a surface such as a glass slide.
What are the advantages of microarray?
The microarrays have become important because they are easier to use, do not require large-scale DNA sequencing and allow the parallel quantification of thousands of genes from multiple samples.
What are the uses of DNA microarray?
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
What does a microarray tell you?
Microarray is a high resolution test to assess very small gains and losses (copy number variants) of genomic information and areas of homozygosity (which might suggest an autosomal recessive condition).
How signal on microarray plate is detected?
The array is washed to remove unbound sample. The array is then scanned to measure fluorescence signal at each spot on the array. The labeled cRNA or cDNA are then hybridized to the microarray, the array is washed and the signal is detected by measuring fluorescence at each spot.