What is non specific DNA-binding?

Nonspecific DNA-binding proteins have evolved to tolerate a wide range of DNA sequences, yet bind with a respectable affinity. The nonspecific binding requirement is in contrast to that imposed on, for example, transcription factors and implies a different structural basis for the biomolecular recognition process.

What is FIS site?

Two RNA polymerase-binding sites and at least six high-affinity Fis-binding sites are present in the fis promoter region. Expression of this fis operon is negatively regulated by Fis in vivo and purified Fis can prevent stable complex formation by RNA polymerase at the fis promoter in vitro.

Are there different types of DNA-binding proteins?

DNA-binding proteins can incorporate such domains as the zinc finger, the helix-turn-helix, and the leucine zipper (among many others) that facilitate binding to nucleic acid. There are also more unusual examples such as transcription activator like effectors.

How do DNA-binding proteins recognize specific DNA sequences?

Basic Requirements for DNA Binding Proteins recognize a particular sequence by having a surface that is chemically complementary to that of the DNA, forming a series of favorable electrostatic and van der Waals interactions between the protein and the base pairs.

What motifs are commonly found in DNA-binding proteins?

The common motifs include the helix-turn-helix, the homeodomain, the leucine zipper, the helix-loop-helix, and zinc fingers of several types. The precise amino acid sequence that is folded into a motif determines the particular DNA sequence that is recognized.

What do DNA binding motifs do?

The DNA binding proteins have a specific folding pattern which is made of repeated nucleotide sequences called as DNA binding motifs. These motifs permit the binding of DNA binding proteins such as transcription factors to the target sequence. It is composed of recognition region and stabilization region.

Is SunGard FIS now?

In 2015, SunGard was acquired by the FIS company. In February 2017, Vista Equity Partners acquired SunGard’s Public Sector and Education businesses from FIS, and renamed and rebranded SunGard Public Sector as Superion by April 2017, while adding SunGard Education to its own PowerSchool holdings.

Is Fidelity A FIS?

FIS is a Fortune 500® company and is a member of Standard & Poor’s 500® Index. The company was founded in 1968 as Systematics™, which was later acquired by ALLTEL Information Services, and then bought by title insurance giant Fidelity National Financial® in 2003, who renamed it Fidelity Information Services (FIS).

What are the three DNA-binding structures?

Although each of these proteins has unique features, most bind to DNA as homodimers or heterodimers and recognize DNA through one of a small number of structural motifs. The common motifs include the helix-turn-helix, the homeodomain, the leucine zipper, the helix-loop-helix, and zinc fingers of several types.

Which of the following is not a DNA binding motif?

5. Which among the following is not a DNA-binding motif? Explanation: Homeobox is not a DNA-binding motif. The DNA sequence that encodes homeodomain is known as the homeobox.

What motifs are commonly found in DNA binding proteins?

What is the function of DNA binding proteins?

Key Points. DNA- and RNA-binding proteins (DRBPs) constitute a significant fraction of cellular proteins and have important roles in cells. Their functions include control of transcription and translation, DNA repair, splicing, apoptosis and mediating stress responses.

Where does the FIS protein bind to DNA?

Binds to hundreds of transcriptionally active and inactive AT-rich sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed: 16963779 ). “E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo.” Cited for: FUNCTION.

What is the role of FIS in DNA inversion?

Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC.

What is the function of FIS in Escherichia coli?

“The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro.” Cited for: FUNCTION. “Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome.” Cited for: FUNCTION, DNA-BINDING.