What is BrdU assay?
Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
What color is BrdU?
BrdU Cell Proliferation Assay Kit 6813
| Product Includes | Quantity (with Count) | Solution Color |
|---|---|---|
| BrdU | 1 x 150 µl | |
| Fixing/denaturing Solution | 2 x 25 ml | |
| BrdU Mouse Detection mAb | 1 x 500 µl | Green |
| Anti-mouse IgG, HRP-Linked Antibody | 1 x 500 µl | Red |
How does EdU assay work?
EdU Cell Proliferation Assay Principle. Cells grown in the presence of 5-EdU incorporate the compound at thymidine bases during S-phase. Fluorophore-labeled azide reacts with the incorporated EdU to allow detection by microscopy or flow cytometry.
How is BrdU used?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation. As a thymidine analog, it is a marker of DNA synthesis.
How is BrdU Incorporated?
BrdU (5-bromo-2-deoxyuridine) is a thymidine analogue which is incorporated into the cells of DNA synthetic phase. Replicating cells undergo DNA synthesis in a highly regulated, S-phase of the cell cycle. Furthermore, loss of control of cell proliferation underlies the pathology of diseases like cancer.
How does BrdU cause mutation?
During S phase of the cell cycle (when DNA replication occurs), BrdU can be incorporated in place of thymidine in newly synthesized DNA molecules of dividing cells. Because BrdU can replace thymidine during DNA replication, it can cause mutations, and its use is therefore potentially a health hazard.
How does the BrdU cell proliferation ELISA kit work?
BrDU Cell proliferation ELISA kit (ab126556) is an indirect ELISA kit for the detection of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase.
How is the absorbance of BrdU related to cell proliferation?
The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation. Specificity/Sensitivity: BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation.
How long does it take to incubate BrdU cells?
Prepare 10X BrdU solution by diluting BrdU 1:100 with cell culture medium. Plate cells in 96-well plate and incubate with respective test substance. Typical seed cell number is 2500–100000 cells/well depending on cell growth rate. Typical incubation time is 1–72 hr.