What does a strong Iki result indicate?
What does a strong IKI result indicate? Substrate is present.
What does IKI assay test for?
IKI assay: detects the presence of starch Benedict’s assay: test for the presence of reducing sugars, such as glucose or maltose.
What happened when pepsin was boiled?
Boiling pepsin denatured the enzyme causing it to have no effect on BAPNA. The substrate is BAPNA, a synthetic peptide, and pepsin usually hydrolyzes polypeptides found in food. The presence of pepsin hydrolyzes polypeptides into peptides and free amino acids.
When using the benedicts assay which color would indicate the most reducing sugar present?
In lab, we used Benedict’s reagent to test for one particular reducing sugar: glucose. Benedict’s reagent starts out aqua-blue. As it is heated in the presence of reducing sugars, it turns yellow to orange. The “hotter” the final color of the reagent, the higher the concentration of reducing sugar.
Which tube do you think will have the highest lipase activity?
Predict Question: Which tube do you think will have the highest lipase activity? Your answer: tube 3 (lipase, deionized water, bile salts, pH 9.0 buffer).
Why is normal exhalation passive quizlet?
Why is normal exhalation passive? Respiratory muscles are relaxing and elastic tissue in the lungs recoils.
What is iodine test for Class 4?
The iodine test is a chemical reaction-based identification test for starch. In this test, iodine and starch form a distinct blue-black colored complex.
What happens to catalase when boiled?
You should have noticed that the boiled potato produced little to no bubbles. This is because the heat degraded the catalase enzyme, making it incapable of processing the hydrogen peroxide.
What happens when enzyme is boiled?
Boiling and Denaturation At temperatures around boiling, the chemical bonds that hold together the structure of enzymes begin to break down. The resulting loss of three-dimensional structure causes enzymes to no longer fit their target substrate molecules, and enzymes entirely stop functioning.
How do you find out if a reducing sugar is present?
To test for the presence of reducing sugars, a food sample is dissolved in boiling water. Next, a small amount of Benedict’s reagent is added and the solution begins to cool. During the next four to 10 minutes, the solution should begin to change colors. If the color changes to blue, then no glucose is present.
Why is it difficult to detect whether lipase?
Why is it difficult to detect whether lipase is active in tube 5? The pH is already very low, so a decrease in pH might be difficult to detect.