What are antibody beads?

Magnetic beads are used for biomagnetic separation procedures to enrich populations of a target cell, protein, or nucleic acid. Sometimes the conjugation method is blamed for poor target recovery, but often the problem is due to a poorly designed separation rack. …

What is a Flag antibody?

The FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. We offer quality antibodies specific to FLAG tag that can be used in a variety of research needs.

What is Flag immunoprecipitation?

Immunoprecipitation (IP) can be used for efficient, high-yield isolation and purification of proteins fused to the FLAG® peptide tag. The immunoprecipitated FLAG®-tagged fusion proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG® peptide.

How do you purify the flag tagged protein?

How to Purify FLAG-tagged Proteins? FLAG-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The FLAG-tagged protein binds to the FLAG-tag specific monoclonal antibody conjugated on an agarose gel.

What is paramagnetic beads?

Paramagnetic particles are the most widely used solid-surface in medical laboratories immunoassay testing equipments, due to ease of manipulation and large surface area. Most paramagnetic beads are made of iron oxide, and are traditionally black or brown.

What kind of beads are magnetic?

Magnetic beads are made up of tiny (20 to 30 nm) particles of iron oxides, such as magnetite (Fe3O4), which give them superparamagnetic properties. Superparamagnetic beads are different to more common ferromagnets in that they exhibit magnetic behavior only in the presence of an external magnetic field.

What is a 3X FLAG tag?

General description. The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

What is a flag label?

A flag label is ideal product solution for identifying products and to distinguish them each other. The name flag label originates from to the “flag element” that is created due to the application of the label. Flag labels are produced on rugged material and are placed around a certain product.

How do you dissolve a FLAG peptide?

Preparation Instructions To prepare a stock solution, dissolve in TBS (10 mM Tris-HCl, 150 mM NaCl, pH 7.4) to a final concentration of 5 mg/mL. Aliquot and store at –20 °C. Repeated freezing and thawing is not recommended.

How big is a flag tag?

1012.0 Da
2. Alternatives to the Flag-tag

Feature Flag® Tag
Size of the tag in Da 1012.0 Da
Affinity matrix Anti Flag® antibody (approx. 150 kDa)
Elution conditions Flag® peptide, low pH, or tag cleavage
Specificity of interaction (KD) 100 nM (3)

How does the flag tag work?

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). A FLAG-tag can be used in many different assays that require recognition by an antibody.