Are HL60 cells adherent?
The Ad-HL60 cells have a more differentiated phenotype than the non-adherent cells from which they were derived, and synthesized 1.88 +/- 0.07 (+/- S.E.M.) pmol 24,25-(OH)2D3/h per 10(6) cells following culture in RPMI-1640 medium containing less than 0.02 nM 1 alpha,25-(OH)2D3.
Where did HL60 cells come from?
Cell line history HL60 is a human cell line derived from peripheral blood lymphocytes of a 36 year old woman suffering from acute promyleocytic leukaemia. It was one of the first human myeloid leukaemia cells to be established as a continuous suspension cell culture which had previously been difficult to achieve.
What are HL60 cells used for?
HL-60 cells can be used for assessing chemotherapy drugs and, when differentiated, can be used as a model for vaccine targets. Other studies of note have used HL-60 cells to look at apoptosis (programmed cell death).
What does HL60 stand for?
human promyelocytic leukemia cell line
HL60 (human promyelocytic leukemia cell line) HL60 is a human caucasian promyelocytic leukemia cell line (Figure 7.3(a)), derived from a 36-year-old woman with acute PML at the National Cancer Institute.
Are platelets myeloid cells?
In hematopoiesis, myeloid or myelogenous cells are blood cells that arise from a progenitor cell for granulocytes, monocytes, erythrocytes, or platelets (the common myeloid progenitor, that is, CMP or CFU-GEMM), or in a narrower sense also often used, specifically from the lineage of the myeloblast (the myelocytes.
Why are HL 60 cells good for stem cell research?
The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process.
What is APL disease?
Acute promyelocytic leukemia (APL) is a blood cancer characterized by a marked increase in a type of white blood cells known as promyelocytes, a type of immature white blood cell. It develops in about 600 to 800 individuals each year in the United States, most often in adults around the age of 40.
What are HL 60 cells and why do they make a good model system for stem cell research?
HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells.
Is myeloma the same as myeloid leukemia?
Multiple myeloma and leukemia are both types of blood cancers but they are not the same disease. Multiple myeloma is a blood cancer that affects plasma cells, which are a certain type of white blood cell. In multiple myeloma, the body produces too many plasma cells in the bone marrow.
What is the most curable leukemia?
While it is similar in many ways to the other subtypes, APL is distinctive and has a very specific treatment regime. Treatment outcomes for APL are very good, and it is considered the most curable type of leukemia. Cure rates are as high as 90%.
Is AML and APL the same?
Acute promyelocytic leukemia (APL) is a unique subtype of acute myeloid leukemia (AML). APL cells have a very specific abnormality that involves chromosomes 15 and 17, leading to the formation of an abnormal fusion gene called PML/RARα. This mutated gene causes many of the features of the disease.
What kind of cell line is HL60?
HL60 (human promyelocytic leukemia cell line) HL60 is a human caucasian promyelocytic leukemia cell line (Figure 7.3 (a)), derived from a 36-year-old woman with acute PML at the National Cancer Institute. HL60 cells are predominantly a neutrophilic promyelocyte, used for laboratory research on how certain kinds of blood cells are formed.
What kind of research is HL 60 used for?
Jump to navigation Jump to search. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology.
How long does it take HL 60 to double?
HL60. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours.
What happens to HL60 cells when exposed to VDDs?
Exposure of either HL60 or NB4 cells to differentiation-inducing concentrations of VDDs causes activation and nuclear translocation of MAPK [48,60]. In these experiments 1,25 (OH) 2 D 3 stimulated the transient (24–48 h) phosphorylation of ERK1/2 in HL60 cells.